Cytometry

The Cytometry and Cell Sorting Facility has the mission of making available to the research community cutting-edge technologies and technical personnel specialized in the field of flow cytometry and cell sorting, offering its services both to IIS-IDIBAPS personnel and to other external entities, especially R&D centers, public or private.To do this, we have a 62m2 cytometry room in which 4 digital cytometers, 1 of them is a full spectrum flow cytometer, and a 44m2 cell sorting room with 2 digital cell sorters.The Facility staff consist of a coordinator, a senior technician and 2 junior technicians; all of them full-time, organized so that the Facility works uninterruptedly from 8h to 20h, to assimilate as many experiments as possible. The staff has proven experience in the field of flow cytometry and cell sorting.We offer a wide range of applications in flow cytometry and develop or implement new ones depending on researchers needs.We provide training, as well as technical knowledge and advice for experimental design, analysis, and data management.

Facility

Images

Equipment

  • BC CytoFlex analyzer cytometer: 6 LASERS. 21 parameters. Plate reader. Volumetric. Absolute counting. APDs.

  • BC CytoFlex cytometer analyzer: 3 lasers. 13 parameters. Plate reader. Volumetric. Absolute count.

  • BD LSRFortessaSORP + HTS cytometer analyser: 4 lasers. 16 fluorescence parameters.

  • BD LSRFortessaSORP cytometer analyser: 5 lasers. 18 fluorescence parameters.

  • Cytek_AURORA cytometer full spectrum analyzer: 5 lasers, plate loader, 40 tube rack.

  • BD FACSARIA II cell separator: 3 lasers. 9 fluorescence parameters.

  • BD FACSAriaSORP cell separator: 5 lasers. 18 fluorescence parameters.

Provisional Contacts

Isabel Crespo |Head of Unit |  icrespo@recerca.clinic.cat  | 932 275 400 Ext.4515 |  ORCID ï»¿

Head of Unit

Isabel Crespo |  icrespo@recerca.clinic.cat  | 932 275 400 Ext.4515 |  ORCID ï»¿

Senior Technician

Cristina López García |  clopezg@recerca.clinic.cat  | 932 275 400 Ext.3364

Specialized Technician

Sara Ozcoz |  ozcoz@recerca.clinic.cat  | 932 275 400 Ext.3364

Aina Rodríguez Vilarrupla, PhD | Core Facilities Director |  aina.rodriguez@idibaps.org  |  ORCID ï»¿

Owners

Centres CERCA List

Equipments & Services Offered

. BC CytoFlex digital bench-top cytometer: 3 LASER, 13 fluorescence parameters, with plate reader. · BD LSRFortessaSORP digital bench-top cytometer: 5 LASER, 18 fluorescence parameters. · BD LSRFortessaSORP digital bench-top cytometer with plate reader: 4 LASER, 16 fluorescence parameters. · BD FACSARIA II digital cell soter: 3 LASER, 9 fluorescence parameters. · BD FACSAriaSORP digital cell sorter: 5 LASER, 18 fluorescence parameters. .Cytek_AURORA full spectrum flow cytometer: 5 LASER, Plate Loader, 40 tubs rack.

Services for IIS-IDIBAPS and other external entities, public or private. All services can be done with the assistance of the Facility staff or unassisted. · Sample analysis by flow cytometry (up to 5 LASER and 18 fluorescence parameters). With HTS module for plate reading. · Cell sorting (up to 5 LASER and 18 fluorescence parameters). Collection in tubes (up to 4 populations), plates or slides. Index single_cell sorting for sequencing (96 and 384 well plates). · Data analysis (DiVa 9 , CellQuestPro 6.0.3, ModFitLT 3.2, FlowJo X, FCAPArray 1.0.1, SpectroFlo, FCS Express, SpectroFlo, Cytobank, CytExpert). · Training for using bench-top cytometers unassisted. · Experimental advice on project/assay development and experimental design.

Categorització

RIS3CAT domains & other

RIS3CAT Domains
CERCA GINYS Categories

GINYS-IDIBAPS-003

Access procedure & Fares

Institutional Networks