Protein technologies

The Biomolecular and Protein Screening Technologies Unit (BMS & PT) offers CRG-CNAG / PRBB researchers, external institutions and private companies a new high-quality technological platform to carry out the production of proteins and automated processes in the context of biomolecular and biochemical screening assays. Through staff-assisted detection, users will have access to facility equipment, including liquid handlers, plate readers, and small molecule libraries. In addition, the unit aims to support CRG institute researchers by purchasing, researching, and maintaining valuable stocks of clones and strains.

Institutional Contacts / Head of Facilities

MM

Provisional Contacts

Carlo Carolis | Head of Unit |  carlo.carolis@crg.eu  |  ORCID  |  PRC Page 

Carlo Carolis |  carlo.carolis@crg.eu  |  ORCID  |  PRC Page 

Mònica Morales (Head of Core Technologies Program) |  monica.morales@crg.eu  | Phone: +34 933160373

CERCA Institutes

Owners

Centres CERCA List

Videos

Equipments & Services Offered

ÄKTA Xpress twin and ÄktaFPLC system for protein purification (GE Healthcare) Biacore T100 (SPR) Monolith NT.115 Thermophoresis (MST) UPLC (shimatzu) – DAWN8 + Optilab® T-rEX ™ LabCHIP GXII TECAN Freedom evo 200 robotic platform Caliper Zephyr robotic platform Caliper Sciclone robotic platform Biotek ELx405 washing machine Kendro incubator MultidropV2 Tecan M200 plate reader Electroporator Amaxa 96-inch plate siRNA libraries The entire human genome collection siGENOME SMARTPoool, (Dharmacon, Thermo-Fisher) consists of groups of 4 different siRNAs per gene and is distributed in four “sets” as follows Kinases – 779 gens GPCR – 516 people “Drugable” genome: 6022 genes Remaining genome collection: 13803 genes Chemical compound libraries The Prestwick Chemical Library®: 1200 small molecules, 100% marketed drugs Prestwick Phytochemical Library – 320 natural products Tocris kinase inhibitor toolbox (Tocris): 80 small molecule compounds

Full service production of plasmid clones, mutants and plasmid libraries. High performance cloning Production of recombinant proteins and protein complexes: bacteria, insect cells and mammalian expression Purification of proteins and antibodies Biophysical characterization of proteins and nucleic acids: Surface plasmon resonance, microscale thermophoresis, size exclusion chromatography – multiangle light scattering Automation of biomolecular and biochemical screening assays Genome editing using the Crispr-Cas9 system: knockout / Knockin gene cell line engineering in collaboration with the CRG Tissue Engineering Unit

Categorització

RIS3CAT domains & other

RIS3CAT Domains
CERCA GINYS Categories

GINYS-CRG-003

Access procedure & Fares

Open – Check conditions.

Papers

Institutional Networks