Cell immortalization

The Cell Immortalization Unit of the Josep Carreras Leukemia Research Institute offers B-cell infection with Epstein-Barr virus (EBV) which leads to further and subsequent immortalization. This is considered the method of choice for generating lymphoblastoid cell lines (LCL). Cell culture is an essential tool for studying the fundamentals of genetic background variables. With the development of personalized medicine, this is increasingly applied to the development and safety testing of drugs. Immortalized cells are derived from primary cells, thus establishing an immortalized cell line to achieve cultured cells in vitro. The purpose of unlimited proliferation and no difference between cells. The Cell Immortalization Unit works closely with you in an integrated manner starting with the project discussion (including the project outline and experimental design). we are continuously working to optimize standard operating procedures and implement the latest methodologies. With our personalized service, you can get our high quality customer service experience with high success rates, frequent communication and fast deadlines.

Institutional Contacts / Head of Facilities

FRANCESC SOLECoordinador Plataformes

Institut de Recerca Contra la Leucèmia Josep Carreras (IJC)

Provisional Contacts

Head of facilities

CERCA Institutes

Badalona, Spain

2010

Institut de Recerca Contra la Leucèmia Josep Carreras (IJC)

Owners

Centres CERCA List

Address

Link

https://www.carrerasresearch.org/ca/unitat-de-cell-immortalization_172651

Equipments & Services Offered

Services

Cell immortalization service to generate cell lines of lymphocytes Produce control material for rare genetic disorders Cryopreservation service to obtain sufficient material from DNA, RNA, and protein samples, which appears to be a valid strategy for further studies.

Categorització

RIS3CAT domains & other

Domains

RIS3CAT Domains

CERCA GINYS Categories

CERCAGINYS code

GINYS-IJC-007

Access procedure & Fares

Access procedure

Open

Availability

Papers

Germline mutations in the spindle assembly checkpoint genes BUB1 and BUB3 are infrequent in familial colorectal cancer and polyposis

Mur P, De Voer RM, Olivera-Salguero R, Rodríguez-Perales S, Pons T, Setién F, Aiza G, Valdés-Mas R, Bertini A, Pineda M, Vreede L, Navarro M, Iglesias S, González S, Brunet J, Valencia A, Esteller M, Lázaro C, Kops GJPL, Urioste M, Puente XS, Capellá G, Valle L.

CERCA GINYS Papers

Germline missense pathogenic variants in the BRCA1 BRCT domain, p. Gly1706Glu and p. Ala1708Glu, increase cellular sensitivity to PARP inhibitor olaparib by a dominant negative effect

Vaclová T, Woods NT, Megías D, Gomez-Lopez S, Setién F, García Bueno JM, Macías JA, Barroso A, Urioste M, Esteller M, Monteiro ANA, Benítez J, Osorio A.

CERCA GINYS Papers

Whole-exome sequencing identifies MDH2 as a new familial paraganglioma gene

Cascón A, Comino-Méndez I, Currás-Freixes M, de Cubas AA, Contreras L, Richter S, Peitzsch M, Mancikova V, Inglada-Pérez L, Pérez-Barrios A, Calatayud M, Azriel S, Villar-Vicente R, Aller J, Setién F, Moran S, Garcia JF, Río-Machín A, Letón R, Gómez-Graña Á, Apellániz-Ruiz M, Roncador G, Esteller M, Rodríguez-Antona C, Satrústegui J, Eisenhofer G, Urioste M, Robledo M.

CERCA GINYS Papers

A mutation in the POT1 gene is responsible for cardiac angiosarcoma in TP53-negative Li-Fraumeni-like families

Calvete O, Martinez P, Garcia-Pavia P, Benitez-Buelga C, Paumard-Hernández B, Fernandez V, Dominguez F, Salas C, Romero-Laorden N, Garcia-Donas J, Carrillo J, Perona R, Triviño JC, Andrés R, Cano JM, Rivera B, Alonso-Pulpon L, Setien F, Esteller M, Rodriguez-Perales S, Bougeard G, Frebourg T, Urioste M, Blasco MA, Benítez

CERCA GINYS Papers

A novel Werner Syndrome mutation: pharmacological treatment by read-through of nonsense mutations and epigenetic therapies

Agrelo R, Sutz MA, Setien F, Aldunate F, Esteller M, Da Costa V, Achenbach

CERCA GINYS Papers

Epigenetic inactivation of the Sotos overgrowth syndrome gene histone methyltransferase NSD1 in human neuroblastoma and glioma

Berdasco M, Ropero S, Setien F, Fraga MF, Lapunzina P, Losson R, Alaminos M, Cheung NK, Rahman N, Esteller

CERCA GINYS Papers

The impact of MECP2 mutations in the expression patterns of Rett syndrome patients

Ballestar E, Ropero S, Alaminos M, Armstrong J, Setien F, Agrelo R, Fraga MF, Herranz M, Avila S, Pineda M, Monros E, Esteller M.

CERCA GINYS Papers

Institutional Quality

HRS4R

Human Resource Strategy for Researchers It supports research institutions and funding organizations in implementing the Charter and Code in their policies and practices. The implementation of the Charter and Code principles by research institutions makes them more attractive to researchers looking for a new employer or host for their research project..

Roger CabezasUnitat de TransferènciaI-CERCA

CERCA GINYS Quality

Code of Conduct CERCA

The CERCA Code of Conduct, aligned with the European Code of Conduct for Research Integrity, recognized by the European institutions, provides guarantees in terms of transparency, open access to data and results, industrial property management, scientific integrity and relationship with the media, among others.

Roger CabezasUnitat de TransferènciaI-CERCA

CERCA GINYS Quality

Institutional Networks

CPTAC - Clinical Proteomic Tumor Analysis Consortium

The Clinical Proteomic Tumor Analysis Consortium (CPTAC) of the National Cancer Institute (NCI-USA) is a national effort to accelerate the understanding of the molecular basis of cancer by applying proteome and genome analysis to large-scale or proteogenomics.

CERCA GINYS Networks

CIBERONC - Cancer

Mission: Promotion of excellence in cancer research in Spain, as well as the incorporation of new findings into clinical practice.

CERCA GINYS Networks

BIOCORES - Health Facilities Directory

BioCores is a directory of basic facilities, technology platforms and scientific services currently in Spain. BioCores offers a tool to find a wide range of cutting-edge, state-of-the-art scientific services, techniques and equipment, to support both biomedical and life sciences research and the scientific community.

CERCA GINYS Networks

REGIC - Network of clinical research management entities

REGIC is the first association of clinical research management organizations created in Spain at the request of professionals, to share experiences and create a space for interaction and training in the management of healthcare R & D & I.

CERCA GINYS Networks