
Flow cytometry platform
Facility
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Main Contacts
JM
Institutional Contacts / Head of Facilities
MM
Provisional Contacts
CERCA Institutes

L'Hospitalet de Llobregat, Spain
2004
Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)
Owners
IDIBELL
Equipments & Services Offered
The unit is equipped with 3 flow cytometers from Beckman Coulter Company: 1x High Speed Cell Sorter Moflo-XDP: 4 lasers (B-R-V-Y), 11 colors (5+2+2+2) o (5+2+4) 2x Gallios Analizers: 3 lasers (B-R-V), 10 colors (5+3+2). Laser abbreviations and Optical Bench configuration: Lasers: V: Violet, 405nm; B: Blue, 488nm; Y: Yellow, 561nm; R: Red, 635nm. Optical Bench Configuration: 10C [2 + 5 + 3] = 10 Colors or PMT detectors in total and [number of them linked to a specific laser line).
Services: Sample acquisition at the analyzer, either with technical support, or without, after receiving a proper training. Cell Sorting with technical support. Data analysis support if required. The aim of the Cytometry Unit is to get ready and focus on the applications of interest of our users. Most common applications that routinely are run: Cell Sorting: In purification mode (when the most important is to get a high purity of the cell population of interest from the original sample). Up to 4 cell populations at the same time (4way sorting). In enrichment mode (when the most important is to get a high recovery of the cell population of interest from the original sample). So that, purity is slightly compromised. Available in 4-way sorting too. In single cell deposition (to get just one single cell or any desired number of cells within a single well using any of the standard multiwelll plates). In mixed mode (purify and enrich mode run simultaneously even for 2 cell populations at a time). Analysis: Multicolor Immunophenotyping Characterization. Viability Assays. Cell Cycle and Proliferation Assays. Gene Expression (Fluorescent Protein analysis). Etc…
Categorització
RIS3CAT domains & other
Social and Health System
Industrial System
Educational and knowledge generation System
TECHNOLOGICAL EQUIPMENT
Citometry
GINYS-IDIBELL-005
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