Flow cytometry platform

Our Flow Cytometry facility provides users with the state-of-the-art equipment for flow Cytometry analysis and high-speed cell sorting. Unit personnel support users for experimental design, data analysis and acquisition. Following manufacturer instructions, a proper maintenance and standard quality control procedures are routinely applied to ensure best quality results.

Facility

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Main Contacts

JM

Jose Miguel Andres VaqueroResponsable Plataforma de Citometría de Flujo - IDIBELL SCTs
Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)

Institutional Contacts / Head of Facilities

Provisional Contacts

José Miguel Andrés Vaquero | Head of Unit |  flowcytometry@idibell.cat  | +34 93 607 38 24 ext3290 |  ORCID  |  PRC Page 

Head of Unit

José Miguel Andrés Vaquero |  flowcytometry@idibell.cat  |  ORCID  |  PRC Page 

CERCA Institutes

Owners

Centres CERCA List

Equipments & Services Offered

The unit is equipped with 3 flow cytometers from Beckman Coulter Company: 1x High Speed Cell Sorter Moflo-XDP: 4 lasers (B-R-V-Y), 11 colors (5+2+2+2) o (5+2+4) 2x Gallios Analizers: 3 lasers (B-R-V), 10 colors (5+3+2). Laser abbreviations and Optical Bench configuration: Lasers: V: Violet, 405nm; B: Blue, 488nm; Y: Yellow, 561nm; R: Red, 635nm. Optical Bench Configuration: 10C [2 + 5 + 3] = 10 Colors or PMT detectors in total and [number of them linked to a specific laser line).

Services: Sample acquisition at the analyzer, either with technical support, or without, after receiving a proper training. Cell Sorting with technical support. Data analysis support if required. The aim of the Cytometry Unit is to get ready and focus on the applications of interest of our users. Most common applications that routinely are run: Cell Sorting: In purification mode (when the most important is to get a high purity of the cell population of interest from the original sample). Up to 4 cell populations at the same time (4way sorting). In enrichment mode (when the most important is to get a high recovery of the cell population of interest from the original sample). So that, purity is slightly compromised. Available in 4-way sorting too. In single cell deposition (to get just one single cell or any desired number of cells within a single well using any of the standard multiwelll plates). In mixed mode (purify and enrich mode run simultaneously even for 2 cell populations at a time). Analysis: Multicolor Immunophenotyping Characterization. Viability Assays. Cell Cycle and Proliferation Assays. Gene Expression (Fluorescent Protein analysis). Etc…

Categorització

RIS3CAT domains & other

RIS3CAT Domains
CERCA GINYS Categories

GINYS-IDIBELL-005

Access procedure & Fares

Open

Institutional Networks