IBUB – Seahorse Oxidative metabolism analysis equipment

The Agilent Seahorse analyzer allows in vitro measurement of the oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR). The OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis as is the ATP production rate, and together these measurements provide a systems-level view of cellular metabolic function.

Institutional Contacts / Head of Facilities

MD

MARTA DIVICOORDINADORA CORE FACILITIES
Institut de Recerca Sant Joan de Déu (IRSJD)

Provisional Contacts

Dr. Aleix Gavaldá | Manager |  aleixgavalda@ub.edu  | 934021549 |  ORCID  |  PRC Page 

Manager

Dr. Aleix Gavaldá |  aleixgavalda@ub.edu  | 934021549 |  ORCID  |  PRC Page 

Marta Divi |  marta.divi@sjd.es  | 603 484 523 , Ext: 80369

CERCA Institutes

Owners

Centres CERCA List

Equipments & Services Offered

Agilent Seahorse XFe24 Analyzer. This equipment provides information on metabolic rates in real time and features a four-port injection system with automated mixing function for real-time detection of live-cell responses to substrates, inhibitors and other pharmacological compounds. The assay is performed in a 24-well plate format.

The samples used in this analyzer may be monolayer or 3D cell cultures, for example: Cell cultures (stable lines, primaries) Organoids Tissue explants or biopsies (ex vivo measurements) Pancreatic islets It may be noted that this technology’s great versatility allows the use of other types of samples to be considered as well.

Categorització

RIS3CAT domains & other

RIS3CAT Domains
CERCA GINYS Categories

GINYS-IRSJD-003

Access procedure & Fares

Self-service, requiring prior certification of the user’s basic training (provided by IBUB), or under scientific cooperation between IBUB and the respective group/organization

Institutional Networks