Molecular interaction platform

The Molecular Interactions Platform provides scientific-technological support to the scientific community in the field of molecular interactions and biomarker discovery using several approaches. First, Surface Plasmon Resonance (SPR) allows real-time monitoring of any type of molecular interactions (protein-protein, protein-drugs, protein-vesicles, protein-RNA, …) and performs high-precision measurements of affinity and kinetic constants, binding specificity, isotyping and screening of potential medications/drugs. Next, Luminex technology allows the detection of up to fifty different biomarkers at once in biological samples and fluids using the technology of magnetic beads. In addition, metabolism and bioenergetics, driving cellular functions such as cell signalling, proliferation, or cell fate, can be measured with the Seahorse in real-time in living cells. Mitochondrial respiration, glycolysis, ATP rate oxidation and fatty acid oxidation can be measured with high precision. Recently we have acquired a high resolution spatial transcriptomics and proteomics platform,CosMx (Nanostring). CosMx SMI enables rapid quantification and visualization of up to 6,000 RNA and 64 validated protein analytes at single cell resolution in biopsies and tissues samples.Our mission is to provide an integrated support, from experimental design, optimization of assay conditions, experiment execution to data analysis and reporting. Our state-of-the art technology together with the large experience and know-how in biomedical research and protein science are at the service of both internal and external researchers.

Facility

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Main Contacts

SB

Sílvia Barceló BatlloriResponsable Plataforma Interaccions Moleculars
Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)

Institutional Contacts / Head of Facilities

Provisional Contacts

Silvia Barceló | Head of Unit |  molecularinteractions@idibell.cat 

CERCA Institutes

Owners

Centres CERCA List

Equipments & Services Offered

CosMx Spatial Multiomics Single-Cell Imaging Platform (Nanostring) SIMOA SR-X ultra-sensitivebiomarkerdetection System (Quanterix) Biacore T-200 (GE) for SPR analysis of interactions Seahorse XFe96 Analyzer (Agilent) for detection of OCR and ECAR in 96-well plate Magpix (Luminex) including xPonent 4.2 software for multiplex analysis of up to 50 magnetic beads Fluorescence scanner Typhoon FLA9500 (GE) equipped with blue, green and red laser

Spatial transcriptomics and proteomics at single cell resolution with CosMx (Nanostring). 6000-RNA, 1000-RNA or 64-protein plex assays in 2- or 4-slide format.Determination of affinity constant (KD), kinetics: association and dissociation constants (kon, koff) in different models. Determination of effective concentration and quality control. Antibody isotyping. Screening of potential drugs/biosimilars Ultra-sensitivequantification of up to 4 biomarkers in biological fluids andsamples in 96-well plate by SIMOA Quantification of up to 50 biomarkers in biological fluids and samples in 96-well plate by Luminex Measure of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in 96-well plate (Seahorse): mitohocondrial respiration, glycolysis, ATP production, lipolysis Fluorescent Scanning of several formats Experimental desing, set up and optimization of experimental conditions Scientific-technological support to researchers Training of new users in using the equipement

Categorització

RIS3CAT domains & other

RIS3CAT Domains
CERCA GINYS Categories

GINYS-IDIBELL-006

Access procedure & Fares

Open

Institutional Networks