Nuclear magnetic resonance

Ultrashield Bruker Avance NEO 300 NMR Spectrometer Bruker Avance 400 Ultrashield NMR Spectrometer Ultrashield Bruker Avance NEO 500 NMR Spectrometer Ultrashield Bruker Avance II 400 NMR Spectrometer Bruker Avance III 500 Ultrashield Plus NMR Spectrometer

1) MONODIMENSIONAL Standard analysis of small molecules -) Standard 1H (proton) experiments -) Standard 13C{1H} (carbon with proton decoupling) experiments -) DEPT/DEPTQ experiments. Carbon experiments with carbon signals separated according to multiplicity. -) 1H and 13C{1H} qNMR (quantitative) experiments. Experiments performed in anyone of the available instruments (300 MHz, 400 MHz or 500 MHz) 2) BIDIMENSIONAL Analysis of medium sized molecules. Molecular mapping -) COSY and TOCSY experiments. 1H – 1H connectivity. -) NOESY and ROESY experiments. 1H spatial orientation. -) HMQC and HSQC experiments. 1H – 13C one-bond connectivity. -) HMBC experiments. 1H – 13C several-bond connectivity. -) HOESY experiments. 1H versus 13C spatial orientations. -) INADEQUATE experiments. 13C – 13C connectivity. Experiments performed in anyone of the available instruments (300 MHz, 400 MHz or 500 MHz) or in the 500 MHz instruments equipped with a cryoprobe (depending on the experiment/sample concentration). 3) MULTINUCLEAR Analysis of complex molecules with heteroatoms. -) Direct acquisition of less common nuclei experiments (31P, 19F, 15N, 11B, 29Si, 195Pt, 119Sn). -) COSY and TOCSY experiments. X – X connectivity (X may be 31P, 19F). -) HMQC, HSQC and HMBC experiments. 1H – X connectivity (X may be 31P, 19F, 15N, 11B, 29Si, 195Pt, 119Sn). -) HMQC, HSQC and HMBC experiments. 19F – X connectivity (X may be 13C, 31P, 15N, 11B, 29Si, 195Pt, 119Sn). -) HMQC, HSQC and HMBC experiments. 31P – X connectivity (X may be 13C, 15N, 11B, 29Si, 195Pt, 119Sn). -) 13C{1HX} experiments. 13C experiments with double decoupling, proton and X (31P or 19F). -) 19F{1H31P} experiments. 19F experiments with double decoupling, proton and phosphorus. -) 31P{1H19F} experiments. 31P experiments with double decoupling, proton and fluorine. Experiments performed either on the 500 MHz instrument equipped with three channels or in the 500 MHz instrument equipped with a cryoprobe. 4) VARIABLE TEMPERATURE/DIFFUSION EXPERIMENTS Study of dynamic/complex systems -) Variable temperature (heating and/or cooling) experiments, on all the available nuclei. From -150 C to + 150 C (if the solvent allows it) -) DOSY (diffusion) experiments on all the available nuclei. Determination of number of species present/diffusion coefficients of molecules. Experiments performed either on the 500 MHz instrument equipped with three channels or in the 500 MHz instrument equipped with a cryoprobe.

GINYS-ICIQ-001

Open