Flow cytometry platform

Analyzer cytometer FACSCalibur (BD). 2 lasers: 4 fluorescence parameters. FACSCanto II (BD). 3 lasers: 8 fluorescence parameters. MACSQuant 10 (Miltenyi Biotec). 3 lasers: 8 fluorescence parameters. Equipped with a magnetic enrichment column. MACSQuant VYB (Miltenyi Biotec). 3 lasers: 8 fluorescence parameters. It is equipped with a yellow-green laser (561nm) and it specializes in bacteria and fluorescent proteins (GFP, mCherry, dTomato…). MACSQuant 16 (Miltenyi Biotec). 3 lasers: 14 fluorescence parameters. Specialized in nanocytometry. Cell sorting FACSAria (BD) inside biosafety cabin. 3 lasers: 9 fluorescence parameters. Collection in cytometry tubes (up to 4 populations), plaque, or slides. Single-cell option. AutoMACS (Miltenyi Biotec). Automated magnetic separation. Multiplex Analysis MAGPIX (Luminex). Analysis of up to 50 analytes per plaque. Off-line analysis programs FACSDiva and Cell Quest Pro. Flow Logic- software for cytometric analysis (Miltenyi). Belysa Immunoassay Curve-Fitting Software (Luminex- Millipore).

The flow cytometry platform has a wide range of applications in flow cytometry and cell sorting and is open to innovation with new techniques at the request of researchers. Support users from experimental design to data analysis to perform proper flow cytometry Sample processing and analysis service for internal and external users Conventional cytometry Characterization of cell populations by multicolor immunophenotyping. Essays of cell viability and proliferation in animal and bacterial cells. Essays of cell cycle and apoptosis. Gene expression (analysis of fluorescent proteins) and quantification of transfection efficiency. Magnetic column cell enrichment for the detection of minority populations. Cell sorter Up to 4 cell subpopulations can be obtained simultaneously in a biosafety cabin. Automated magnetic cell separation of up to two populations. High Throughput Screening (HTS) Analysis of gene expression and immunophenotype profiles, detection of DNA, RNA and protein in a single plaque. Possibility to include up to 50 analytes in the same plaque.

GINYS-IRSP-004

Open. Check requirements with the platform staff