Proteomics

As a reference proteomics center, the Proteomics Unit has state-of-the-art equipment including high-performance liquid chromatography systems and high-resolution Orbitrap mass spectrometers to identify and quantify proteins of interest. The instruments available in the Proteomics Unit cover the most advanced techniques in the field of proteomics, and are accessible to national and international scientists.

Characterization of proteome interactions Identification of protein-protein interactions Identification of protein-DNA interactions Identification of protein-RNA interactions Characterization of post-translational modifications Identification and quantification of acetylations, methylations, and ubiquinations in purified proteins Identification and quantification of post-translational modifications in purified histones. Identification and quantification of phosphopeptides Characterization of complete proteomes Proteome quantification (with and without label – TMT, SILAC, etc.) Phosphoproteome quantification (with and without label – TMT, SILAC, etc.) Proteome characterization of liquid biopsies (plasma, cerebrospinal fluid) Analysis of a single protein Confirmation of overexpressed proteins in complex samples Identification of a protein in gel bands Molecular Weigh determination of intact proteins Targeted proteomics Peptide selection for biomarker quantification using targeted proteomics Optimization of targeted proteomics methods for biomarker quantification Quantification of biomarkers using targeted proteomics RNA and DNA modifications Analysis of modified (ribo-)nucleosides by mass spectrometry

GINYS-CRG-004

Open. Procedure