Spectroscopy and characterization of materials

The High Resolution Mass Spectrometry Unit (HRMS) offers access to state-of-the-art mass spectrometry instruments capable of characterizing the wide range of compounds that are usually of interest to the Institute. The apparatus includes most of the ionization options, introduction of chromatographic samples and techniques that are necessary to solve problems of mass spectrometry in chemistry. In addition to the characterization of pure samples, the unit also supports research projects that depend more on mass spectrometry experiments, such as mechanical studies, intermediate identification, characterization of impurities or biochemical applications. In addition, the Unit offers a comprehensive training to guarantee an optimal use of the installations and an adequate interpretation of the data.

Facility

Images

Fluorescence microwell plate reader

icroplate reader FLUOstar Omega. System composed by a xenon flash-lamp, wheels with 8 excitation and 8 emission filters, temperature control from ambient +5ºC to 45ºC, a reagent injector and a PMT detector. The instrument allows fluorescence and absorbance intensity measurements in the UV/Vis in microplates up to 1536-wells. Wavelength range for fluorescence: 240-740 nm. Wavelength range for absorbance: 220-850 nm.

UV-Vis-NIR spectrophotometer

UV-Vis-NIR spectrophotometer Lambda 1050 from Perkin Elmer. This high performance instrument has double beam optics, double monocromator and three detectors system (PMT, InGaAs and PbS) covering 175 to 3300 nm range.

UV-Vis Spectrophotometer III

Double beam UV-Vis Shimadzu spectrophotometer model UV-1800. Spectral range from 190 to 1100 nm, 1 nm fixed slit.

UV-Vis Spectrophotometer II

Double beam UV-Vis Agilent Technologies spectrophotometer model Cary 60 UV-Vis. Spectral range from 190 to 1100nm, 1.5nm fixed slit and room light immunity.

UV-Vis Spectrophotometer I

Double beam UV-Vis Shimadzu spectrophotometer model UV-2401PC with a Peltier thermostated (7-60 ºC) sample holder. Optical range from 190 to 1100 nm, variable slit.

Time Correlated Single Photon Counting (TCSPC) Spectrometer

LifeSpec II from Edinburgh Instruments. This is a compact, high performance, computer controlled spectrometer for the rapid measurement of fluorescence lifetimes based on the technique of Time Correlated Single Photon Counting. The LifeSpec II has two detectors, one standard PMT in the UV-vis range and another PMT nitrogen cooled with spectral response in the vis-NIR region from 300 to 1700nm.

Fluorescence spectrometer

Fluorescence spectrometer OmniFluo900 Series from Zolix equipped with a PMT detector and continuum Xe light source.

Spectrofluorimeter II

Spectrofluorimeter Horiba Jobin Yvon. System composed by a continuum 450W Xenon lamp, double monochromator for excitation, a solid sample holder, detection in Right Angle or Front Face mode and absorbance measurements. The instrument allows fluorescence measurements in the wavelengths range of the UV/Vis and NIR from 250 to 1600 nm.

Portable Raman Spectrometer 785nm

Dispersive Raman Spectrometer model i-Raman from BWTEK. This instrument has an excitation laser at 785nm and a fiber optic probe with a TE Cooled Linear Array detector. The resolution is 3cm-1 and the spectral range goes from 75 to 3200cm-1.

Portable Raman Spectrometer 532nm

Dispersive Raman Spectrometer model i-Raman from BWTEK. This instrument has an excitation laser at 532nm and a fiber optic probe with a TE Cooled Linear Array detector. The resolution is 3cm-1 and the spectral range goes from 75 to 4000cm-1.

Polarimeter

Polarimeter Jasco model P-1030 with simmetrical angular oscilation for the Sodium line and photomultiplier tube detector. Angular range: ± 90 º, speed better than 6 º s-1.

FTIR spectrometer I

FTIR spectrometer Nicolet is50 from Thermo Scientific, with DLaTGS and MCT detectors, and FTRaman module with the capability of Raman mapping and well plates screening. The instrument also includes a built in ATR module with diamond crystal that allows measuring samples in the Far Infrared (up to 150 cm-1) and Middle Infra-red regions (4000-400cm-1). Spectral range in main sample compartment: 4000–400 cm-1.

FTIR spectrometer II

FTIR Bruker spectrometer model Alpha with an ATR accessory. Spectral range from 4000 to 400 cm-1.

FTIR spectrometer III

FTIR React IR 15 from Mettler Toledo. The instrument allows in situ reaction monitoring in the mid-infrared. Spectral range: 3000 to 650 cm-1.

FTIR spectrometer IV

FTIR spectrometer Cary 630 from Agilent technologies equipped a DLaTGs detector (4 cm-1 resolution) and a single bounce diamond ATR accessory (spectral range 650-4000cm-1). There is also a DialPath accessory for measuring liquid samples in transmission mode with selectable 30, 50 and 100 μm pathlength.

Bruker EMX X-band Micro EPR

EMX Micro spectrometer with an X-band bridge of 9.1 – 9.9 GHz. Allows acquiring spectra of both solution and powder samples for studying metal complexes or organic radicals at ambient conditions (298 K) or low temperature like 4 K (He) or 77 K (N2), in perpendicular or parallel mode. Additionally, paramagnetic compounds can be generated in situ via UV-Vis irradiation (Hg lamp 200 – 2000 nm) or external devices (LEDs).

Brookfield Viscometer DV-II+pro

Fluid viscosity at given shear rates

Anton Paar Rheometer MCR302

Allows determining the absolute viscosity and the flow behavior under different conditions of speed or shear stress and temperature.

ZEN3600 NanoZS

Malvern Light scattering (DLS) analyzer for nanoparticles and macromolecules in suspension. Hydrodynamic diameter by DLS (0.6 to 6000 nm). Zeta potential of particles between 5 and 10000 nm by DLS

Quantachrome micro Ultrapyc 1200e

Allows measuring the true or skeletal density of solids (without considering the volume occupied by pores) by gas displacement, normally helium. Precision increases with the amount of sample (ideally 0.5-2 cm3).

Pfeiffer Omnistar mass spectrometer

Online analysis of gases. Up to 100 AMU. Analysis of gas streams and atmospheres. Support of other apparatus (i.e., setups of the researchers).

Quantachrome Autosorb iQ gas adsorption analyser

Porous structure of solids by gas physisorption (BET area, pore volume and pore size distribution) typically by nitrogen. Argon also available.

Institutional Contacts / Head of Facilities

Provisional Contacts

Dr. Georgiana Stoica | Responsible |  gstoica@iciq.es  | 977 920 200 (ext. 352)

Technician

Mariona Urtasun |  murtasun@iciq.es  | 977 920 200 (ext. 352)

Responsible

Dr. Georgiana Stoica |  gstoica@iciq.es  | 977 920 200 (ext. 352)

Dr. Gisela Colet | Facilities Coordinator |  gcolet@iciq.cat  | Phone: +34 977 920 238

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Equipments & Services Offered

Elute UHPLC-timsTOF flex™ mass spectrometer (Bruker Daltonics) ESI/MALDI dual source and Coldspray Ionization coupled to ion mobility (IM). Coldspray ionization-Ion Mobility-MS: Unique combination for the analysis of ionic compounds at low temperature (up to -100 ºC), including separation of isomeric ions. MALDI Imaging – Ion Mobility-MS: Isomers separation / Isobaric ions. Determination of ions on surfaces. Liquid chromatograph coupled time-of-flight mass spectrometer Waters Alliance HPLC coupled to MicroTOF Focus (Bruker Daltonics) with electrospray (ESI) and atmospheric pressure chemical ionization (APCI) options. Liquid chromatograph coupled time-of-flight mass spectrometer Waters LCT Premier liquid chromatograph coupled time-of-flight mass spectrometer (HPLC/MS-TOF) with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) options. MALDI time-of-flight mass spectrometer BRUKER Autoflex matrix assisted laser desorption ionization (MALDI) time-of-flight mass spectrometer. Liquid chromatograph coupled quadrupole-time-of-flight mass spectrometer. (UPLC-QqTOF). Waters liquid chromatograph (UPLC) coupled to MaXis Impact (Bruker Daltonics) hybrid analyzer: quadrupole-time-of-flight mass spectrometer (UPLC/MS-QqTOF) with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) options.

Autoflex™ mass spectrometer: MALDI-TOF-MS m/z up to 70,000 Peptides and Proteins, Polymers, Oligonucleotides Low molecular weight molecules MaXis Impact™mass spectrometer (Bruker Daltonics) with ESI and APCI ionization sources. Coupled to an Acquity™ UPLC (Waters). ESI-QqTOF-MS ESI: Polar molecules, high and low molecular weight compounds. Proteins Organometallics. Non-Covalent Interactions MS/MS analysis. APCI- QqTOF-MS Medium Polarity Compounds, m/z up to 1500 MicroTOF™ mass spectrometer (Bruker Daltonics) with ESI, APCI and Coldspray ionization sources. Coupled to an Ultimate3000™ HPLC (Thermo Scientific). TOF-MS Coldspray ionization: analysis of ionic compounds at low temperature (until -100 ºC). Labile species, organometallic compounds, reaction intermediates. ESI/MALDI dual source and Coldspray Ionization coupled to ion mobility (IM) Coldspray ionization-Ion Mobility-MS: Unique combination for the analysis of ionic compounds at low temperature (up to -100 ºC), including separation of isomers. MALDI Imaging – Ion Mobility-MS: Isomers separation / Isobaric ions. Localization of ions on tissues.

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