UTBM – Biological and molecular techniques unit

The Biological and Molecular Techniques Unit (BMTU) is specialized in the molecular analysis of a wide variety of aqueous matrices (human consumption sources, river biofilm, sediments, wastewater, activated sludge) from high quality extraction of nucleic acids (DNA and RNA). The application of molecular techniques such as PCR and qPCR allows the detection and quantification of any target gene in the user’s samples. The unit (BMTU) has specialized in the quantification of resistance genes from a wide range of antibiotic families (ARGs), in agreement with the compounds detected by the UEM, promoting the transversality of the laboratories that compose the SCT. The BMTU also has a Radioactive Facility with non-encapsulated sources. This allows the study of 3H, 14C, 35S radionuclides by using an equipment recommended for samples that require a high level of sensitivity.

Facility

Images

Large volumen centrifuge, Avanti J-26 XPI

Scintillation equipment, Tricarb 2910 TR, Perkin-Elmer

Molecular Imager, Gel Doc Xr+, Bio-rad

Homogenizer, FastPrep 24, MP Biomedicals

Microtox, Model 500, SDI Environmental

Quantitative Thermal Cycler PCR, CFX96, Bio-rad

Conventional Thermal Cycler PCR, 2720, Applied Biosystems

Gradient Thermal Cycler PCR, Veriti, Applied Biosystems

Qubit, 4.0, Invitrogen

Refrigerated centrifuge, 5804 R, Eppendorf

Institutional Contacts / Head of Facilities

SI

Sara Insa

Institut Català de Recerca de l’Aigua (ICRA)

Provisional Contacts

Main Contact(s) of Facility

Staff

Head of facilities

CERCA Institutes

Girona, Spain

2006

Institut Català de Recerca de l’Aigua (ICRA)

Owners

Centres CERCA List

Address

Link

https://icra.cat/en/serveis-cientfics-i-/biological-and-molecular-techniques-unit-utbm/

Equipments & Services Offered

Equipments

High performance centrifuge, Avanti J-26 XPI, Beckman Coulter. Low activity liquid scintillation analyzer, TriCarb 2910 TR, Perkin-Elmer. Gel Documentation System, GelDoc Xr+, Bio-rad. Cytometer, Attune NxT Azul, Fisher Scientific. Ultrafreezer, TDE Series -86ºC (422L), Fisher Scientific. Homogenizer, FastPrep 24, MP Biomedicals. Microtox, Model 500, SDI Environmental. Quantitative PCR thermal cycler, CFX96, Bio-rad. Conventional PCR thermal cycler, 2720, Applied Biosystems. Gradient PCR thermal cycler, Veriti, Applied Biosystems. Qubit fluorometer, 2.0, Invitrogen.

Services

Study of bacterial communities Characterization and quantification of gene elements related to anthropogenic activity Characterization and quantification of the resistome Acute toxicity (microtox) and mutagenicity (Ames test) analysis in water samples Analysis of radioisotopes (3H, 14C, 35S) Sample storage service at -80ºC

Categorització

RIS3CAT domains & other

Domains

RIS3CAT Domains

CERCA GINYS Categories

CERCAGINYS code

GINYS-ICRA-003

Access procedure & Fares

Access procedure

Contact to the responsible for the BMTU: Àlex Sànchez (asanchez@icra.cat)

Availability

Official fares & Access

https://icra.cat/wp-content/uploads/2022/07/ID_005_Politica_qualitat_v1.pdf

Official fares & Access

https://ginys.cerca.cat/wp-content/uploads/2023/01/Tarifes_GINYS-ICRA-003_1-2.pdf

Institutional Quality

HRS4R

Human Resource Strategy for Researchers It supports research institutions and funding organizations in implementing the Charter and Code in their policies and practices. The implementation of the Charter and Code principles by research institutions makes them more attractive to researchers looking for a new employer or host for their research project..

Roger CabezasUnitat de TransferènciaI-CERCA

CERCA GINYS Quality

Code of Conduct CERCA

The CERCA Code of Conduct, aligned with the European Code of Conduct for Research Integrity, recognized by the European institutions, provides guarantees in terms of transparency, open access to data and results, industrial property management, scientific integrity and relationship with the media, among others.

Roger CabezasUnitat de TransferènciaI-CERCA

CERCA GINYS Quality