
UAT – Cytometry platforms
Main Contacts
RP
Institutional Contacts / Head of Facilities
MR
Provisional Contacts
CERCA Institutes

Barcelona, Spain
2002
Vall d’Hebron Institut de Recerca (VHIR)
Owners
VHIR
Equipments & Services Offered
Facs Aria Digital Cell Sorter (BD): 4 lasers to detect up to 12 fluorochromes simultaneously. Two FacsCalibur (BD) cell analyzers: 2 lasers for simultaneous detection of up to 4 fluorochromes. Fortessa LSR (BD) cell analyzer: 4 lasers and parallel detection of up to 16 fluorochromes. Acquisition of high performance samples (96 and 384 well plates). Data analysis software: FCS Express (online licenses) and FlowJo (installed on a UAT computer).
Cell analysis by flow cytometry • FACS (Fluorescence-Activated Cell Sorting), for the isolation of specific cells or populations. The UAT offers advice on experimental design and data analysis, technical support and general training in cytometry, including the handling of self-service cytometers. Tailor-made experiments (including labeling) can be carried out on request, after consultation with Unit staff. APLICATIONS • Immunophenotyping based on the labeling of surface and intracellular markers • Studies derived from the labeling of nucleic acids (ploidy, cell cycle) • Functional analysis (enzymatic activity, cell integrity, membrane permeability, calcium fluxes) • Identification and isolation of cell populations or particles (cell classification)
Categorització
RIS3CAT domains & other
Social and Health System
Industrial System
Educational and knowledge generation System
TECHNOLOGICAL EQUIPMENT
Citometry
GINYS-VHIR-001
Access procedure & Fares
Open. Consult the conditions
Medium
Institutional Quality
Institutional Networks